Search
Now showing items 1-3 of 3
In-house expression and purification of Phusion DNA polymerase for high fidelity polymerase chain reaction (PCR) amplification.
(Makerere University, 2022-04)
Background: DNA polymerases are indispensable tools in the molecular biology field during synthesis of DNA molecules in the Polymerase Chain Reaction (PCR). Phusion DNA polymerase engineered from a processivity enhancing ...
In-house expression and purification of phusion DNA polymerase for high fidelity polymerase chain reaction. (pcr) amplification
(Makerere University, 2022-04-05)
Background: DNA polymerases are indispensable tools in the molecular biology field during synthesis of DNA molecules in the Polymerase Chain Reaction (PCR). Phusion DNA polymerase engineered from a processivity enhancing ...
Expression and purification of an in-house Phusion DNA Polymerase using Escherichia coli BL21 (DE3) cells
(Makerere University, 2022)
Introduction: DNA polymerases are enzymes that play a major role in enabling scientists to replicate DNA from organisms for a variety of studies in Molecular Biology. For years these polymerases have been isolated from a ...