Pre-clinical validation and formulation of capsules of abelmoschus esculentus seed extract for weight loss.

Abstract

ABSTRACT Back ground; Abelmoschus esculentus (Okra) is a vegetable that is consumed worldwide and has been studied and found to be rich in phytochemicals like flavonoids, polyphenols etc. Various studies about the plant have proved its medicinal importance in conditions like diabetes mellitus, hyper-lipidemia and obesity among others. Over 315million people are affected by obesity and it’s putting them at a risk of developing chronic conditions like cardiovascular diseases. The current medications e.g. orlistat are associated with high cost and intolerable adverse effects like faecal incontinence. The use of locally available natural agents to curb obesity would be of tremendous benefit Objective: To pre-clinically validate weight loss activity of Abelmoschus esculentus seed extract and formulate capsules of seed extract to aid in weight loss Method: An experimental study design was used in the study. Dry Abelmoschus esculentus seeds were ground and extracted using 70% ethanol. Weight loss activity of the extract was tested in hamster albino rats by first inducing overweight with cheese and then orally administering different doses of the extract for 24 days. Weights were taken every 3 days to assess weight loss. Acute toxicity and histopathological studies done to assess the safety of the extract. From the extract, capsules were formulated and their physical properties, dissolution, uniformity of weight and stability profile were determined using appropriate methods from the British Pharmacopoeia (2009). Results: The extract induced significant weight loss (P value less than 0.05) which was greater than that caused by the positive control orlistat. The acute toxicity and histopathological studies showed no serious effects on the organs (kidneys, liver and heart). The formulated capsules passed the quality control and stability tests. Conclusion: Abelmoschus esculentus seed extract causes weight reduction, is safe for use and can easily be formulated into capsules of acceptable quality to aid in weight loss