Show simple item record

dc.contributor.authorBiira, Peace Audrey
dc.date.accessioned2023-01-24T10:17:58Z
dc.date.available2023-01-24T10:17:58Z
dc.date.issued2022-10
dc.identifier.citationBiira, P.A. (2022). Comparing carbapenamase genes in e. coli isolated from clinical and environmental sources submitted to the ncbi. (MakUD). Unpublished Undergraduate Report. Makerere University, Kampala, Uganda.en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12281/14758
dc.descriptionA research dissertation submitted to the Department of Plant Sciences, Microbiology and Biotechnology, College of Natural Sciences, in partial fulfillment of the requirements for the award of the degree of Bachelor of Science in Biotechnology of Makerere University.en_US
dc.description.abstractAntibiotic resistance is the condition when bacteria can survive and multiply in the presence of various antibiotics. The emergence of resistance to carbapenem class of drugs has become a major global public health crisis. Bacteria have carbapenemases (beta-lactamases) that inhibit almost all beta-lactam drugs including the carbapenemases that represent the most diverse hydrolytic potential. These carbapenemases have been studied and sequenced from across the world and the different sequences have been submitted to data bases for reference during different studies. They are classified into four different classes which include, class A, B, C and D. In this study we will compare samples from different countries submitted to the NCBI to establish their relationship and the dominant class.The first step was mining of these sequences from NCBI data base and they were saved on notepad. A total number of 30 were collected plus an outgroup sequence. The data collected was represented in form of a table. Using MEGA 11 software, sequence alignment was done using clustal W to reflect charges in each sequence. Modal selection was then done and a phylogenetic tree was constructed. The prevalent class of carbapenemases is class A and there is an evolutionary relationship among these carbapenemases. The tree is rooted showing they share a common ancestor. There is also a high divergence among them from the root across different countries showing the high mutation rates among these genes. The study has showed that carbapenemases are widely spread across the world and the prevalent class is A. Class B have the lowest assurance. There is also high mutation rates among the E. coli giving them an advantage and becoming more of a threat to human health hence more efforts and research should be put in antibacterial resistance among microorganisms to come up with a solution to this great problem.en_US
dc.language.isoenen_US
dc.publisherMakerere university.en_US
dc.subjectCarbapenamase genes in e.coli.en_US
dc.subjectAntibiotic resistanceen_US
dc.subjectcarbapenemases (beta-lactamases)en_US
dc.subjectBeta-lactam drugsen_US
dc.subjectE.coli.en_US
dc.titleComparing carbapenamase genes in e. coli isolated from clinical and environmental sources submitted to the ncbi.en_US
dc.typeTechnical Reporten_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record