Prevalence of CTX-M antimicrobial resistance gene in enterobacteraceae isolates that were collected in Microbiology Laboratory College of Health Sciences, Mulago.

Abstract

Aim: To determine the prevalence of CTX-M antimicrobial resistance gene in enterobacteriaceae isolates collected from Microbiology laboratory, College of Health Sciences Mulago. Samples: Archived Enterobacteriaceae isolates Study design: Laboratory based cross-sectional study Methods: Enterobacteriaceae clinical isolates were sub cultured, and identified using biochemical tests. They were screened for ESBL and confirmed by double disc synergy. Genotyping was performed using the convetional PCR for CTX-M gene and agarose gel electrophoresis to detect amplification. A subset of 4 amplicons was later sequenced by Sanger sequencing Technology. Results: In the present study, molecular methods are used to evaluate the prevalence of the extended-spectrum beta-lactamase (ESBL)-encoding CTX-M gene among Enterobacteriacea strains. In total, 57 clinical samples were collected from microbiology laboratory after Disc synergy diffusion method was used to detect the presence of ESBL production. Moreover, antibiotic resistance patterns and molecular detection of CTX-M ESBLs, were also studied. The DNA isolated from the 57 samples included Escherichia coli (n=3, 5%) Klebsiella sp. (n=10, 18%), and other species (n=44, 77%). Genotypic detection of CTX-M gene by PCR revealed (n=21, 37%) positive for CTX-M gene of which (n=3, 14%) Klebsiella pneumoniae and (n=1, 5%) of E.coli harbored CTX-M gene. Generally, a high resistance rate is observed against 3GCs. Lower resistance is observed against carbapenems. Our study showed that ESBLs producers were resistant to commonly used antibiotics. However ESBL production by CTX-M gene is still less prevalent in our clinical isolates.