Development of a biosensor system that tests For the levels of aflatoxin in groundnuts.

Abstract

With the discovery of aflatoxins, a lot of study has been done with the goal of identifying these toxins in contaminated food and feed because of their carcinogenic effect. The majority of traditional detection approaches are time-consuming, with complex separation processes, including complicated multi-step procedures that necessitate sample destruction for precise toxin determination. With more regulations for acceptable levels of aflatoxins in place, modern techniques can now produce results with extremely high precision and accuracy, making them suitable for regulatory laboratories and post-harvest sample testing in developed nations. Unfortunately, a great deal of the world's affected nations lacks ready access to high performance liquid chromatography and mass spectrometry equipment. This necessitating the development of alternative, easily accessible, and straightforward detection techniques that can be used by agrodealers and small-scale farmers in developing nations. This proposal report presents an overview of the existing detection and quantification methods for aflatoxins. It also covers the traditional, quantitative, chemically-based analytical strategies for detecting aflatoxins in groundnuts and their evolution to the modern instrumentation routinely used in developed countries.