Prevalence of extended spectrum beta lactamase (ctxm and tem) drug resistant genes among eschelishia coli isolates from Entebbe Uganda.

Abstract

Introduction Existence of drug resistant genes is major constraint in the treatment and management of patients infected with E. coli and has facilitated the continued spread of diseases in the communities and increased expenditure on health. Objective The objective of the study was to determine the prevalence of extended beta lactamases (CTXM and TEM) drug resistance genes among E. coli isolates from Entebbe Uganda. Methods It was a cross sectional study that examined presence or absence of drug resistant genes among E. coli isolates from Entebbe in Uganda. Isolates already known to be E. coli were given to the group for growth and multiplication after which analysis on presence or absence of drug resistant genes was done. Bacterial deoxyribonucleic acid (DNA) was extracted using Quiagem (Germany) following acceptable procedures and analyzed for presence or absence of drug resistant genes using polymerase chain reaction Primers of size 800bp for blaTEM and 600bp for blaCTXM were used. Data was visualized under an illuminator using ultra violet light for formation of bands indicating presence of the genes if interest. Results A total of 32 E. coli isolates were analyzed out of which 1 (one) was positive for presence of blaCTXM genes and 7 (seven) were positive for presence of TEM genes giving a prevalence of 3.13% for blaCTXM and 21.9% for blaTEM genes among the study isolates analyzed. Conclusion Existence of drug resistant genes in E. coli is real and a major public health concern which negatively affects response to treatment leading to reduced manhours and increased expenditure on health at national and personal level.