In-vitro determination of antibacterial activity of Cannabis Sativa against staphylococcus aureusIn-vitro determination of antibacterial activity of Cannabis Sativa against staphylococcus aureus

Abstract

Medicinal plants are important for animal and human health care and widely used for the treatment of different infectious diseases caused by bacteria with a large impact on public health. This study was aimed to determine the in vitro antibacterial activity of cannabis sativa. This study was conducted to determine the antibacterial activity of cannabis sativa aqueous and ethanol extracts and to determine synergism between cannabis sativa and erythromycin, a commercial antibiotic. The antibacterial activity was determined by agar well diffusion method, organic extract had pronounced activity (22.3mm), positive control had a largest diameter (26.6mm), aqueous extract (18.3mm), organic + erythromycin (23.6mm), aqueous + erythromycin (20.2mm) and 50% ethanol (solvent) did not show any activity. The MIC was determined by two fold dilutions of both extracts using broth microdilution method. The MIC of organic extract was compared to that of aqueous extract. Further research should be done to identify the exact active compounds that can be employed to control wide range of diseases.