Prevalence of the TEM gene among Eschericia coli and Klebseilla pneumoniae isolates from microbiology laboratory

Abstract

Background: Extended spectrum beta lactamases play a key role in antimicrobial resistance by inhibiting the beta lactams such as the cephalosporins and penicillins preventing the drugs from killing the Enterobacteriaceae. This creates a burden to both the healthcare professionals and the health sector due to increased mortality and morbidity. Objective: The study was aimed at determining the prevalence of the TEM gene in the selected Escherichia coli and Klebsiella pneumoniae isolates from Microbiology laboratory College of Health Sciences Makerere University. Methodology: 46 known isolates of Escherichia coli and Klebsiella pneumoniae were obtained from the clinical microbiology laboratory, DNA was extracted from the isolates using the conventional CTAB method. Conventional PCR method was applied to amplify the TEM gene. Results: Out of the 46 isolates, 17 (36.97%) were positive for the TEM gene accounting for the and the of the 29 (63.03%) were negative. Conclusion: The prevalence of the TEM gene indicated a worrying percentage that calls for urgent attention from the healthcare professionals and ministry of health for effective treatment of Enterobacteriaceae infections through drug development and sensitizing people against self-medication.