Evaluation of anti bacterial activity of ethanolic crude leaf extracts of mangifera indica L AND biden pilosa against methicillin resistant staphylococcus aureus.

Abstract

Background: 80% of the world population depended on natural herbal medicines for primary health care. In Uganda, the National Drug Authority and policy recognized medicinal herbs as being a vital component to saving human lives for their pharmacological activities since they supplemented synthetic medicines. Bidens pilosa and Mangifera indica are herbal plants known to be effective against staphylococcus species and other infectious diseases such as gastroenteritis, septicemia and wound infections. Methicillin resistant staphylococcus aureus (MRSA) a superbug in the healthcare system is known to cause nosocomial infections in hospitals as well as Nursing home care facilities such as pneumonia, bacteremia, septicemia, endocarditic and toxic shock syndrome. The activity of the combined ethanolic crude leaf extract of Mangifera indica and Bidens pilosa against these bacteria were determined in this study. Objectives: To evaluate the anti-bacterial activity of combined ethanolic crude leaf extracts of Bidens pilosa and Mangifera indica with the individual extracts against MRSA. Todetermine the MICs of individual ethanolic crude leaf extracts of Bidens pilosa and Mangifera indica collected from around Makerere University main campus against MRSA. To assess if the combined ethanoic crude leaf extracts of the Bidens pilosa and Mangifera indica had antibacterial activity against MRSA. To determine which ratio of the combination had the highest antibacterial activity against the MRSA. Method An experimental study design, Well-diffusion method on a resistant isolate. Conclusion: This study combined the effects of these two extracts with evidence that the combination was superior than the effects of individual extract.