Assessing the efficacy of aloe vera extract (barbadensis) and guava extract (psidium guajava) on xanthomonas campestris pv campestris at conas microbiology laboratory

Abstract

Bacterial diseases in plants have significant impacts on agricultural production worldwide. Xanthomonas campestris pv. Campestris is a Gram negative bacterium(Bonas et al., 2000) that causes a variety of diseases in plants, including bacteria spot diseases in tomatoes and black rot diseases in cruciferous vegetables. The control of this pathogen is challenging due to the development of resistance to the conventional chemical treatments(Itako et al., 2015). Therefore there is a growing interest in exploring alternative methods such as the use of plant extracts with antibacterial properties. Aloe Vera and guava are two plants known for their medicinal properties, including antibacterial activity. Several studies have reported the efficacy of Aloe Vera and guava extracts in inhibiting the growth of various bacterial pathogens. For example ,Aloe Vera extract has been shown to have antibacterial activity against Staphylococcus aureus and Escherichia coli(Azahra et al., 2019). while guava leaf extract has been found to inhibit the growth of Salmonella typhi and S.aureus(Biswas et al., 2013). This project is to find out the efficacy of Aloe Vera extract and Guava extract on Xanthomonas campestris pv. Campestris, a bacterial pathogen that causes diseases in a variety of plants. The research will involve conducting in the vitro experiments to assess the antimicrobial properties of these extracts against the pathogen. The study will involve the extraction of Aloe Vera and Guava extracts using ethanol of different concentrations. The extracts will then be tested against Xanthomonas campestris pv. Campestris to evaluate their inhibitory effects on bacterial growth. The results of this project which is inhibiting the growth of Xanthomonas campestris pv. Campestris will provide valuable insights into potential use of Aloe Vera and Guava extracts as natural antimicrobial agents for controlling Xanthomonas campestris pv. Campestris Infections.