Prevalence of CTXM gene in isolates of enterobacteriaceae collected from the Microbiology Laboratory

Abstract

Multi drug resistance in Enterobacteriaceae is a common cause of hospital and community acquired infections. Enterobacteriaceae are gram negative bacteria like E. coli, Klebsiella Pneumoniae are pathogenic organisms causing many infections like pyelonephritis in pregnant women, neonatal sepsis in babies, blood stream infections which are usually acquired in hospitals. These bacteria can be treated with antibiotics like fluoroquinolones, aminoglycosides, beta lactams, and macrolides. However, bacteria developed resistance to these drugs. For years Carbapenems have been used successfully to treat infections due to resistant Enterobacteriaceae including those producing Extended Spectrum Beta lactamases (ESBLs).The most frequent illnesses in both hospital and community-based settings are bacterial infections and the poor clinical results have been attributed to Extended Spectrum Beta-Lactamase Producing Enterobacteriaceae leading to antibiotic resistance. However, we are unsure of how dire the situation is in actual communities thus the need to determine the prevalence. This study aimed at determining the prevalence of CTX-M gene in Enterobacteriaceae isolates obtained from the Microbiology Laboratory. Objective The general objective was to determine the prevalence of the CTX-M gene from isolates of Enterobacteriaceae in the microbiology laboratory Methods A laboratory based cross-sectional study was carried out among Enterobacteriaceae isolates obtained from the Microbiology Laboratory. Bacterial DNA was extracted from the isolates, genetic detection of the CTX-M using PCR and quantification of the extracted DNA was done using Agarose gel electrophoresis.