dc.contributor.author | Muheereza, Emmanuel | |
dc.date.accessioned | 2023-04-05T11:58:15Z | |
dc.date.available | 2023-04-05T11:58:15Z | |
dc.date.issued | 2022-12-12 | |
dc.identifier.citation | Muheereza, E. (2022). The antimicrobial activity of root extracts of Zingiber Officinale on clinical isolates of Escherichia Coli and Staphylococcus Aureus. (Unpublished Undergraduate Research Project Report). Makerere University, Kampala, Uganda. | en_US |
dc.identifier.uri | http://hdl.handle.net/20.500.12281/15906 | |
dc.description | A research project report submitted to the College of Veterinary Medicine, Animal Resources and Biosecurity in partial fulfillment of the requirements for the award of the degree of Bachelor of Biomedical Laboratory Technology of Makerere University. | en_US |
dc.description.abstract | The grievous outcomes of antimicrobial resistance (AMR) in society support the need
for discovery of better alternative treatments to be used for infections management.
Plants are known potential sources of antimicrobial agents therefore this study
evaluated the antibacterial activity of Zinger officinale on clinical Escherichia coli and
Staphylococcus aureus isolates. Aqueous and ethanolic extracts were obtained from
fresh Z. officinale rhizomes. A total of 49 E. coli and 50 S. aureus isolates were tested
with a panel of medically relevant drugs (trimethoprim sulphamethoxazole, neomycin,
ampicillin, chloramphenicol, ciprofloxacin, gentamicin, ceftriaxone, doxycycline,
penicillin and tetracycline) to establish the resistance patterns. A resazurin-based
micro-broth dilution method was used to determine the minimum inhibition
concentrations (MIC) of the extracts against the bacteria. The prevalence of multidrug
resistance was higher in S. aureus (70%, n =35) compared to E. coli (59.2%, n =29).
Escherichia coli had the lowest average MIC for both extracts (aqueous extract:
152.3mg/ml; ethanolic: 134.7mg/ml). Staphylococcus aureus had the highest mean
MIC for both extracts (aqueous extracts: 198mg/ml; ethanol extract: 172.5mg/ml). The
ethanolic extract demonstrated significantly better mean MIC results on E. coli than on
S. aureus (p<0.05). The ethanolic extract was also found to contain terpenoids which
were absent in aqueous extract. Both ethanol and aqueous extracts contained
considerably similar quantities of flavonoids. The ethanolic extract was more effective
than aqueous extract and E. coli was more tolerant to the extracts. Overall, Z. officinale
is a promising candidate as an alternative to the conventional drugs to be used as
therapeutic agent against clinical microbes. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Makerere University | en_US |
dc.subject | antimicrobial activity | en_US |
dc.subject | antimicrobial resistance | en_US |
dc.subject | Zingiber Officinale | en_US |
dc.subject | root extracts | en_US |
dc.subject | clinical isolates | en_US |
dc.subject | Escherichia Coli | en_US |
dc.subject | Staphylococcus Aureus | en_US |
dc.subject | E.coli | en_US |
dc.title | The antimicrobial activity of root extracts of Zingiber Officinale on clinical isolates of Escherichia Coli and Staphylococcus Aureus | en_US |
dc.type | Thesis | en_US |