| dc.description.abstract | Dudu-acelamectin is a crop pesticide misused by some farmers in Uganda for tick control. The farmers who use it allege that it causes adverse effects on livestock and on human who get exposed to it during the spraying. Therefore, acute and subacute dermal toxicity studies were conducted based on OECD 402 (2017) and OECD 410 (1981) guidelines to assess the allegations on the toxicity of Dudu-acelamectin via dermal route. A total of 24 Wistar Albino Rats were used, where Dudu-Acelamectin was applied on about 10𝑐𝑚2 of shaved skin. In the acute toxicity study, 3 rats were used per dose level. The rats in each group were weighed on day 1, 7 and 14. A dose-range finding study at 200, 1000 and 2000 mg/Kg was conducted where rats were exposed once to the given dose and then observed for clinical signs and mortalities for 14 subsequent days. For the subacute toxicity study, 3 rats per dose were weighed on day 1, 7, 14 and 21, and were tested daily for 21 days at 1, 100, and 200mg/Kg to determine the clinical signs, histo pathological lesions, and the defects on renal and hepatic function tests. The mean values of body weights and the renal and hepatic function tests were statistically analysed, relative to the negative control, using One-way ANOVA at a significance level of 0.05. It was found that Dudu-Acelamectin has a dermal LD50 > 2000 mg/Kg in wistar albino rats. Mild dermal irritation was noted at 1000 and 2000 mg/Kg. Lethargy was observed at 200, 1000, and 2000 mg/Kg. On Day 1, the mean body weight was statistically significant (P 2000 mg/Kg, and causes damages to the skin, liver and kidneys in Wistar Albino Rats. Hence, these adverse effects should be used as experimental evidence of the adverse effects of Dudu-Acelamectin upon dermal exposure in Wistar Albino Rats | en_US |
