Antibacterial activity of Ozoroa insignis against Staphylococcus aureus.

Abstract

This study investigated the antibacterial potential of Ozoroa insignis against Staphylococcus aureus using the zone of inhibition and minimum inhibitory concentration (MIC) assays. Methanol extracts from different parts of the plant (bark, leaf, and stem char) were tested. The zone of inhibition results revealed mean diameters of the bark extract at 26.67±1.366mm, leaf extract at 27.17±1.472mm and stem char at 14.83±2.317mm, all of 0.5g/ml concentration, compared to 47.17±5.529mm for the ampicillin antibiotic positive control of 0.025mg/L concentration. The bark extract exhibited a MIC mean of 12,300.00±264.575µg/ml, the leaf extract had a MIC mean of 12,100.00±100.000µg/ml, and the stem char extract had an MIC mean of 59,700.00±608.276µg/ml. The leaf extract displayed the most significant antibacterial activity, as indicated by its larger zone of inhibition and lower MIC, followed by the bark extract. Stem char extract showed comparatively weaker activity. These findings suggest that Ozoroa insignis extracts, particularly the leaf extract, possess notable antibacterial potential against Staphylococcus aureus, warranting further exploration for its use as a potential preservative and therapeutic application.