Screening of Schelerotium rot root resistant bean lines for Fusarium spp resistance at Nacrri in Wakiso district

Abstract

Fusarium root rot (Fusarium solani f.sp. phaseoli) is one of the most important disease affecting common beans (Phaseolus vulgaris L). This research was conducted at National Crops Resources Research Institute (NaCRRI) to determine the bean lines resistant to Fusarium spp and the virulence of the Fusarium spp isolates. A complete randomized experimental design was used with two replications of each treatment. There were two factors under investigation which are Fusarium spp isolates F890 and F386. Thirty (30) treatments comprising 30 selected bean varieties were used in the study. Each experimental unit comprised of 10 sampling units. Thirty accessions for the study were randomly selected from those that were found to have moderate to high tolerance to Schlerotium rolfsii f. sp phaseoli the causal agent for Schlerotium root rot disease. Disease evaluation was assessed basing on the symptoms of the hypocotyl and roots then scored using the CIAT scale of 1-9. Data analysis was done using GenStat and Micro-Soft Excel computer programmes to obtain differences in the mean disease severity. Analysis of Variance (ANOVA) and t-Test for paired means were used to address the study objectives. The findings of this study revealed that Fusarium spp isolate F890 had a significant effect on genotypes ADP 98, ADP 112, ADP 43, ADP 20, ADP 1, Lira U00030, Lira U00205 and NABE 17 obtained from NaCRRI in Wakiso District at 5 percent level of significance. On the other hand, ANOVA results also showed that Fusarium spp isolate F386 had a significant effect on genotypes ADP 98, ADP 110, ADP 20, ADP 12, ALB 153, NABE 17 and KWP 9. Furthermore, t-test results from the experiment showed that there is no significant difference in the virulence of the two Fusarium spp isolates F890 and F386 at 5 percent level of significance.