Development of a tlc method for control of tetracycline residues in milk sold for consumption in kawempe division
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ABSTACT Background:The intensification of animal production has necessitated the raising levels of antimicrobial use in production, with consequent presence of residues in animal related products. The rising levels of tetracycline use especially in dairy animals for the treatment of mastitis warrant development of robust and rapid screening methods to control these residues and to protect the public against effects associated with tetracycline residues such as antimicrobial resistance and delayed hypersensitivity reactions. Objective: The general objective of this research wasto develop a TLC method for screening for tetracycline residues in milk sold for consumption in Kawempe division assess. Methods Method development involved selection of a mobile phase, extraction, plate development and visualization. All these parameters were optimized and validated on robustness, repeatability, specificity and limit of detection as per the ICH guidelines. During sample analysis, a total of 57 milk samples were collected and extracted as per the developed method, and analysis done as per the method. Visualization was under UV lamp at 366nm Results A TLC method for screening tetracycline residues in milk was developed and validated as per ICH guidelines. The method was developed using a mobile phase (methanol:citric acid:acetone, 10:5:5v/v/v) on TLC glass plates SIL-G F25 with saturation time of 30 minutes and UV detection at 366nm. The Rf values for doxycycline, oxytetracycline and tetracycline were 0.68, 0.59 and 0.6. The method was repeatable, robust with good specificity. All the 57 milk samples analyzed had no detectable levels of tetracyclines. Conclusion The developed TLC method is suitable for use as a preliminary screening tool, to supplement the quantitative methods currently being used. This will reduce on the sampling time and hasten the process of controlling drug residues.