| dc.description.abstract | The aim of this experiment was to investigate the wound healing activity of the roots of C. indica. This was done against the Neomycin sulfate a wound-healing drug on the market as the positive control and the non-treated animal models. The experiment proceeded with use rats as animal models, the ethanolic and the distilled water extracts of C. indica. The objectives of this experiment were; to investigate the phytochemical composition of both water and ethanolic root extracts of C. indica, to investigate the wound recovery ability of the root extract of C. indica, and to compare the wound healing effect between the water and ethanolic extract of C. indica.
The roots of C. indica were collected from Kawanda at 6.30am, dried under direct sunlight and ground at National Chemotherapeutics Laboratory. Two solvents were used separately to get two extracts i.e. 80% ethanol and distilled water by cold maceration and cold maceration then dried the concentrates in a hot air oven at 47°c, and a 10%w/v concentration was made in all the extracts. 16 Rats were divided into make four groups i.e. 1, 2, 3, and 4 treated with ethanolic extract, Neomycin sulfate, aqueous extract and the untreated one respectively. Wounds were incised on healthy rats, by clipping off the fur using a clipped, locally anesthetized with 1% of 1 ml lidocaine intradermally. A well-measured size using a Vernier calliper, demarcated using an ink pen was cut with a scalpel blade and forceps. Then treated was done after 30 minutes and daily for 19 days with measurements taken after 3 days. The parameters were wound area, percentage reduction of the area. Results were analysed by Graph pad prism two way ANOVA and tested with the Tukey’s test.
Both the water and ethanolic extracts of the roots of C. indica showed high concentration of phenolic compounds, flavonoids, alkaloids, glycosides and saponins; however, the ethanolic extract had extra concentration in alkaloids and glycosides. The root extract of C. indica demonstrated an excellent wound healing activity with reference to the Neomycin sulphate treatment a wound healing drug on market, however, the ethanolic extract of C. indica showed a higher wound healing activity than the aqueous extract.
The crude extracts of the roots of C. indica showed a high wound healing potential; therefore, further studies should be done for the preparation of a purified root extract of C. indica. | en_US |
