Development of an in-house COVID-19 RNA extraction kit at the Genomics and Molecular Biology Unit- Laboratory at Makerere University College of Health Sciences

Abstract

This project report details the comprehensive development of an innovative in-house RNA extraction kit, aimed at addressing the growing demand for efficient and cost-effective RNA isolation methods in molecular biology and diagnostics. Traditional RNA extraction kits are often expensive and may not meet specific research needs, prompting the need for a customizable, in-house solution. My project focused on the design, optimization, and validation of an in-house RNA extraction kit. Key components of the report include the formulation and testing of proprietary RNA extraction reagents, the engineering of a user-friendly protocol, and the evaluation of kit performance.