dc.description.abstract | Background: Multi drug resistance in enterobacteriaceae is a common cause of hospital and
community acquired infections. Enterobacteriaceae are gram negative bacteria like E.coli,
Klebsiella Pneumoniae are pathogenic organisms causing many infections like pyelonephritis in
pregnant women, neonatal sepsis in babies, blood stream infections which are usually acquired in
hospitals. These bacteria can be treated with antibiotics like fluoroquinolones, aminoglycosides,
beta lactams, and macrolides. However, bacteria developed resistance to these drugs. For years
Carbapenems have been used successfully to treat infections due to resistant Enterobacteriaceae
including those producing Extended Spectrum Beta lactamases (ESBLs). Misuse of
Carbapenems has led to the emergence of resistance in gram negative bacteria.
Objective: The general objective was to determine the prevalence of multidrug resistance (ESBL
(CTX-M U) and Carbapenemase (NDM and OXA-48) among archived enterobacteriaceae from
patients in Mulago National Referral hospital Maternity ward.
Methodology: This was a cross sectional laboratory-based prospective study. The DNA of the
archived samples used was extracted using boiling lysis method and the samples selected by
random sampling method. Conventional PCR was used during amplification and agarose gel
electrophoresis carried out to visualize the bands of the different genes.
Results: Out of 44 archived samples, 0% prevalence was detected for NDM and OXA-48 genes
with n=0 for E.coli, n=0 for Klebsiella pneumoniae and n=0 for Enterobacter species. However
out of the 44 samples, 64% prevalence (P) for CTX-M U was obtained where Klebsiella
Pneumoniae with 71%(P=17/24) prevalence was the most predominant for CTX-M U gene
followed by Enterobacter species with 64%(P=7/11) prevalence and the E.coli with 44%
prevalence for CTX-M U gene(P=4/9).
Conclusion: This study showed 64% prevalence of ESBL gene (CTX-M U) a great threat to
public health and 0% of Carbapenemase encoding genes (NDM and OXA-48). Implying
infection control measures particularly compliance with hand hygiene guidelines, as well as
appropriate antibiotic policies in order to prevent the ever-increasing problem of emergence of
MDR ESBL and Carbapenemase producing gram negative organisms. | en_US |