The prevalence of Escherichia Coli to third generation cephalosporins from Makerere University clinical microbiology laboratory specimen

Abstract

Introduction: The emergence of extended-spectrum beta-lactamase Enterobacteriaceae is of great concern: a phenomenon that worries clinicians and other healthcare workers due to the serious threat they pose to patients. Therefore, this study was carried out to determine the prevalence of Escherichia coli resistance to third-generation cephalosporins from Makerere University clinical microbiology laboratory specimen. Methods: In this cross-sectional study, we examined 37 isolates of Escherichia coli received in the Clinical Microbiology Laboratory from June 2022 to August 2022. Samples were subjected to antimicrobial susceptibility testing using the disk diffusion method according to the Laboratory standard operating procedures. The ESBL production was tested by the Double Disc Synergy Test (DDST) by using a disc of Augmentin along with three third generation cephalosporins. Data was then entered into Microsoft Excel and analyzed using descriptive and analytical statistics.