Browsing School of Bio-Medical Sciences (Bio-Medical) by Type
Now showing items 41-60 of 109
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Development and evaluation of an in-house RNA Extraction kit for severe acute respiratory syndrome Corona Virus 2(SARS-COV-2)
(Makerere University, 2023)Major objective of the study: To develop an in-house RNA extraction kit for extraction of high quality RNA. Specific objectives of the study: To determine the reagents required to develop the in-house RNA Extraction ... -
Development and evaluation of in-house SARS-COV-2 RNA extraction kit at Genomics Molecular Biology Laboratory, College of Health Sciences, Makerere University
(Makerere University, 2023)Objective: The purpose of this research was to develope in-house RNA extraction kit for SARCOV-2 using readily available reagents at Genomics Molecular biology Laboratory. Methodology: The study was an applied experimental ... -
Development of a hand-made DNA extraction kit for bacterial samples in the genomic and molecular laboratory - Makerere University College of Health Sciences
(Mekerere University, 2023-05)DNA extraction is a critical step in molecular biology research, enabling the analysis of genetic material from various organisms. The development of efficient and reliable DNA extraction methods is essential for accurate ... -
Development of a home-made DNA extraction kit for bacterial samples in the Genomic and Molecular Laboratory - Makerere University College of Health Sciences
(Makerere University, 2023)Background: DNA extraction is a critical step in molecular biology research, enabling the analysis of genetic material from various organisms. The development of efficient and reliable DNA extraction methods is essential ... -
Development of an in-house COVID-19 RNA extraction kit at the Genomics and Molecular Biology Unit- Laboratory at Makerere University College of Health Sciences
(Makerere University, 2023)This project report details the comprehensive development of an innovative in-house RNA extraction kit, aimed at addressing the growing demand for efficient and cost-effective RNA isolation methods in molecular biology and ... -
Effect of laboratory errors on the accurate and timely reporting of bloodstream and wound infections caused by extended spectrum beta-lactamase positive escherichia.coli.
(Makerere University, 2022-01-22)Production of extended spectrum beta lactamase enzymes is one of the common mechanisms of resistance of Escherichia coli to antibiotics, thus timely detection and reporting of this phenotype is very critical and crucial ... -
Evaluation of high-level aminoglycoside resistance of enterococcal isolates in blood cultures at Makerere University clinical microbiology laboratory between 2017 to 2020
(Makerere University, 2022-03)Background: Resistance to aminoglycoside has been of great concern, since it eliminates the synergy of aminoglycosides with beta lactam antibiotics, which is the therapy of choice for most of enterococcal infection which ... -
Evaluation of the diagnostic yield of cell blocks in suspected malignant pleural effusions at Makerere University Core Pathology Laboratory
(Makerere University, 2022-09)Introduction: Tumors that arise from the mesothelium or metastasizing to the mesothelium cause malignant pleural effusion. The most frequent and critical diagnosis to be made or ruled out in undetected unilateral pleural ... -
Expression and purification of an in-house Phusion DNA Polymerase using Escherichia coli BL21 (DE3) cells
(Makerere University, 2022)Introduction: DNA polymerases are enzymes that play a major role in enabling scientists to replicate DNA from organisms for a variety of studies in Molecular Biology. For years these polymerases have been isolated from a ... -
Expression and purification of phusion DNA polymerase from Escherichia coli BL21-DE3 at the molecular biology laboratory of Makerere university biomedical research center
(Makerere university, 2022-03-31)Background Polymerase Chain Reaction (PCR) based assays are considered a gold standard diagnostic tool with broad application in genetic testing (Chau et al., 2020). Thermostable DNA polymerases are particularly important ... -
Expression and purification of phusion polymerase from BL21 (DE3) cells of Escherichia Coli B strains at the Genomics, Molecular and Immunoloy laboratory at Makerere University College of Health Sciences.
(Makerere University, 2022-03-31)Phusion polymerase enzyme is a combination of a proofreading polymerase and a processivity-enhancing domain developed by Finn zymes, resulting in one of the most reliable and robust polymerases available (Westburg, 2021). ... -
Fine needle aspiration cytology findings of breast lumps in female patients presenting with palpable breast masses at Pathology Department Makerere University College of Health Sciences
(2019-09-12)Background: A palpable breast lump is a common clinical problem that presents to surgeons, gynaecologists and general practitioners. In the past, excisional biopsy was the accepted practice current practices utilize ... -
In-house expression and purification of phusion DNA polymerase for high fidelity polymerase chain reaction. (pcr) amplification
(Makerere University, 2022-04-05)Background: DNA polymerases are indispensable tools in the molecular biology field during synthesis of DNA molecules in the Polymerase Chain Reaction (PCR). Phusion DNA polymerase engineered from a processivity enhancing ... -
In-house expression and purification of Phusion DNA polymerase for high fidelity polymerase chain reaction (PCR) amplification.
(Makerere University, 2022-04)Background: DNA polymerases are indispensable tools in the molecular biology field during synthesis of DNA molecules in the Polymerase Chain Reaction (PCR). Phusion DNA polymerase engineered from a processivity enhancing ... -
Infant warmer bag: a device designed to maintain the baby's normal body temperature during hypothermic conditions.
(Makerere University, 2022-09-23)Every year, over 20 million low birth weight and preterm babies are born in Uganda (Byaruhanga R, 2005) Of the 20 million births, 4 million babies die within their first 28 days of life majorly because of the inability ... -
Infection prevention and control in Mulago National Referral Hospital after the third wave of Covid-19
(Makerere University, 2022-09)Background On the global scale out of every 100 patients in acute care hospitals seven patients in high income countries and 15 patients in law and middle-income countries will acquire at least one nosocomial infection ... -
Knowledge, attitude, and practices of mothers/caregivers towards immunization of children under 5 years of age in Awach Health Centre IV, Gulu District.
(Makerere University, 2023-02-25)Introduction: Immunization is a key approach that can be successfully employed to reduce deaths and illnesses among children. Immunization presently averts an estimated 3 million Deaths annually for children aged under ... -
A low-cost manually operated physiotherapy device for alleviation of limb weakness due to prolonged bedrest
(Makerere University, 2022-09)This report encompasses the technical aspects of the activities performed by team MEDI-MINDS up to design III. The contents here in are divided into three sections: Section I-Review of Design I, Section II- Revision of ... -
Management of uterine atony induced post-partum hemorrhage
(MAKERERE UNIVERSITY, 2022-05-18)Postpartum hemorrhage (PPH) is one of the greatest and persistent global health burdens in maternal health attributed to high morbidity and mortality, uterine atony is the highest etiology of PPH, other causes include; ... -
Methicillin-resistant Staphylococcus aureus isolates from clinical samples received at Makerere University Clinical Microbiology Laboratory from 2017 to 2020.
(Makerere University, 2021-12)Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is highly prevalent among patients admitted to hospital wards, and is on the rise as a community associated infection. Laboratory surveillance of distribution ...