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Expression and purification of phusion DNA polymerase from Escherichia coli BL21-DE3 at the molecular biology laboratory of Makerere university biomedical research center
(Makerere university, 2022-03-31)
Background
Polymerase Chain Reaction (PCR) based assays are considered a gold standard diagnostic tool with broad application in genetic testing (Chau et al., 2020). Thermostable DNA polymerases are particularly important ...