Isolation and characterization of amylase producing bacteria from Kabaka's lake
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Since industrial amylase is usually extracted from bacteria and fungi, it is mandatory to isolate a local high amylase producing strain. The aim of the current study was to isolate and characterize an amylase producing bacteria from the water samples collected from Kabaka’s Lake in Uganda. The isolation was done by serial dilution and plating method. Total five bacterial colonies were isolated from the collected water samples. All isolates were screened for amylolytic activity by starch agar plate method. Among three bacterial isolates, only two isolates showed the amylolytic activity. Out of these, one isolate was selected on the basis of big zone of hydrolysis around colony for further study. The isolate was characterized morphologically, physiologically and biochemically. It swas found to be an aerobic, gram positive and rod shaped bacteria. It showed a temperature of 37oC for growth and pH optimum at 7.0. It utilized a wide range of carbon and nitrogen sources.