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dc.contributor.authorKakoowe, Emmanuel
dc.date.accessioned2023-01-23T03:55:08Z
dc.date.available2023-01-23T03:55:08Z
dc.date.issued2022-03-30
dc.identifier.citationKakoowe, E. (2022). Development of Mint-Flavored Probiotic Obushera using Lactobacillus rhamnosus Yoba 2012 [unpublished undergraduate thesis]. Makerere University, Kampalaen_US
dc.identifier.urihttp://hdl.handle.net/20.500.12281/14621
dc.descriptionA Research Project Report Submitted to the Department of Food Science and Nutrition in Partial Fulfilment of the Requirements for the Award of the Degree of Bachelor of Science in Food Science and Technology of Makerere Universityen_US
dc.description.abstractMint essential oil is a common flavorant in the food industry. However, the antimicrobial action of oil is undesirable in production of probiotic foods. In addition, the strong mint flavor can deter the sensory acceptability of the product. This study evaluated the effect of mint essential oil on the viability of Lactobacillus rhamnosus Yoba 2012 and consumer acceptability of the mint-flavored Obushera. Obushera is a traditional fermented sorghum-based beverage from Uganda. The beverage was flavored with 0, 1, 2 and 4% mint emulsion and fermented using Lactobacillus rhamnosus Yoba 2012 (LRY) at 24℃ for 24 h. Microbial counts, pH and titratable acidity (TA) were determined at 0 and 24 h of fermentation. Thereafter, consumer acceptability of the Obushera with ≤ 6 log cfu/mL of the probiotic at 24 h of fermentation was done. Although the Obushera was spiked with about 6 log cfu/mL of LRY at 0 h of fermentation, it was noted that counts reduced with increasing mint concentration. The counts were 5.6, 5.3, 4.8 and 4.0 log cfu/mL in the 0, 1, 2 and 4% mint-flavored Obushera, respectively. At 24 h of fermentation, LRY counts 7.9, 7.8, 7.5 and 5.2 log cfu/mL in the 0, 1, 2 and 4% mint-flavored Obushera, respectively. The pH dropped from pH 5.1 to ˂4.5 in 0, 1 and 2% mint-flavored Obushera in 24 h of fermentation. The TA increased from 0.1 to 0.3% in the 0 and 1% Obushera and 0.1 to 0.2% in the 2% mint-flavored one. The pH and TA of the 4% mint remained the same (pH=5, TA=0.1) throughout the fermentation. The consumer acceptability scores of the 0, 1 and 2% mint-flavored Obushera were in the order 7.2, 5.7 and 4.8, respectively. Results implied that an acceptable probiotic Obushera flavored with ≤1% mint emulsion could be made without adversely inhibiting the probiotic.en_US
dc.language.isoenen_US
dc.publisherMakerere Universityen_US
dc.subjectMinten_US
dc.subjectFlavorsen_US
dc.subjectObusheraen_US
dc.subjectEnturireen_US
dc.subjectLactobacillus rhamnosusen_US
dc.subjectYobaen_US
dc.titleDevelopment of Mint-Flavored Probiotic Obushera using Lactobacillus rhamnosus Yoba 2012en_US
dc.typeThesisen_US


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