School of Bio-Medical Sciences (Bio-Medical): Recent submissions
Now showing items 61-80 of 116
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Comparison between combined disk test and double disc synergy test in detecting extended spectrum beta-lactamases in Klebisiela Pneumoniae
(Makerere University, 2022-08)Introduction: The spread of ESBL producing Klebisiella pneumoniae are causing more severe infections and multidrug resistance because of their continuous mutation making treatment with antibiotics difficult. Therefore there ... -
Colistin micro broth disk elution method in determining susceptibility to colistin in acinetobacter species
(Makerere University, 2022-09)Colistin use as a drug of last resort to treat multidrug-resistant organisms in humans has increased following its re-introduction. This has led to large patterns of resistance among various gram negative bacterial organisms ... -
Assessment of womens’ knowlegde, attitude and beliefs towards cervical cancer screening in Nakaseke Health Center, Central Uganda
(Makerere University, 2020)Cervical Cancer is the second commonest cancer affecting the women, where virtually all cases are connected to human papillomavirus genital infection. Women living in urban centers take cervical cancer infection as bad ... -
Antimicrobial susceptibility profiles of gram negative rods isolated from ICU patients' samples in Mulago Hospital
(Makerere University, 2021-02)Monitoring the prevalence and susceptibility profiles of these GNB is key in empirical therapy as it will guide physicians on which specific antibiotics to administer to these patients with minimal or no drug resistance ... -
qepA gene detection among archived Quinolone resistant uropathogenic Escherichia coli isolates obtained from Microbiology Laboratory, Makerere University, Uganda.
(Makerere University, 2022-02-24)World over, plasmid-mediated quinolone resistance in Escherichia coli clinical isolates is a serious challenge. This is worsened by non-robust surveillance and a scarcity of databases about region-specific determinants of ... -
Prevalence of extended spectrum beta lactamase (CTX-M and TEM) drug resistant genes among Klebsiella pneumoniae and Eschelishia coli isolates from Entebbe, Uganda.
(Makerere University, 2022-03)Background: Antimicrobial resistance rates vary globally and change from time to time. ESBLs are enzymes capable of hydrolyzing and therefore inactivating beta lactam antimicrobials such as penicillin, Cephalosporins and ... -
Carriage of NDM & IMP Carbapenem resistance genes among Escherichia coli & Klebsiela pneumoniae human isolates from Mbarara
(Makerere University, 2022-01)Introduction Carbapenem, a β-lactam that is really potent against Gram- negative bacteria, has been recognized as a last resort for treating of infections caused by multidrug-resistant bacteria. However, the increasing ... -
Carriage of ndm & imp carbapenem resistance genes among e. coli & k. pneumoniae isolates from Mbarara.
(Makerere University, 2022-01)The first reports of carbapenem resistance in Enterobacteriaceae isolates occurred in the early 1990s. Researchers published the first report of an isolate that produced Klebsiella pneumoniae carbapenemase in 2001. Antimicrobial ... -
Prevalence of ESBL genes (CTXM and TEM) among E.coli isolates from Entebbe Regional Referral Hospital.
(Makerere University, 2022-03-30). In this study, the prevalence of ESBL genes (CTXMand TEM) amongE.coliisolates obtained from Entebbe regional referral hospital has been investigated. 32emulsified stool e.coli isolates were collected from patients not ... -
Carriage of ndm & imp carbapenem resistance genes among e. coli & k. pneumoniae human stool isolates from Mbarara
(Makerere university, 2022-03)Background The purpose of the study is to establish the prevalence of NDM & IMP Carbapenem resistance genes among E. coli and K. pneumonia. The study is to be conducted on the isolates from Mbarara at the College of ... -
Expression and purification of phusion polymerase from BL21 (DE3) cells of Escherichia Coli B strains at the Genomics, Molecular and Immunoloy laboratory at Makerere University College of Health Sciences.
(Makerere University, 2022-03-31)Phusion polymerase enzyme is a combination of a proofreading polymerase and a processivity-enhancing domain developed by Finn zymes, resulting in one of the most reliable and robust polymerases available (Westburg, 2021). ... -
Detection of NDM and OXA 48 genes in E. coli and K. pneumoniae isolates from Mulago National Referral Hospital.
(Makerere University, 2022-03)E. coli and K. pneumoniae are part of the normal flora in the human body. When an individual gets immunocompromised, these organisms can cause human infections as gastrointestinal infections, UTIs, pneumonia and meningitis ... -
Prevalance of extended spectrum beta-lactamases among escherichia coli and klebsiella pneumoniae in isolates from Mulago National Referral Hospital.
(Makerere University, 2022-03)Escherichia coli (E.coli) and Klebsiella pneumoniae are gram-negative bacteria that are members of the enterobacteriaceae family of bacteria. Antimicrobial resistance is one of the most serious risks to global public health. ... -
Assessing the prevalence of malaria among children under 5 years, attending to treatment in Kamuli general hospital- Kamuli Municipality
(Makerere University, 2021-01)Malaria is a life threatening disease typically transmitted to humans through female anopheles mosquito carrying the parasite and remains one of the top killers of children below five years despite of the nationwide mosquito ... -
Prevalence of extended spectrum beta-lactamases in e. coli and k. pneumoniae isolates from Mulago National Referral hospital.
(Makerere University, 2022-03)Extended-spectrum Beta-lactamases (ESBLs) are most often a plasmid-mediated heterogeneous group of β-lactamase enzymes, that confer resistance to a wide range of commonly used β-lactam antibiotics including third-generation ... -
Expression and purification of phusion DNA polymerase from Escherichia coli BL21-DE3 at the molecular biology laboratory of Makerere university biomedical research center
(Makerere university, 2022-03-31)Background Polymerase Chain Reaction (PCR) based assays are considered a gold standard diagnostic tool with broad application in genetic testing (Chau et al., 2020). Thermostable DNA polymerases are particularly important ... -
Carriage of NDM and IPM carbapenem resistance genes among E.coli and K.pneumoniae isolates from Mbarara
(Makerere University, 2022-03)Background: The increasing prevalence of carbapenem-resistant Enterobacteriaceae strains is related to high mortality and it is increasing around the world. Among the Enterobacteriaceae family, Klebsiella pneumoniae and ... -
Prevalence of quinolone resistant gene qep in uropathogenic escherechia coli isolates from female patient samples at the Microbiology Laboratory Makerere University College of Health Sciences
(Makerere Univeristy, 2022-01)Background Drug resistant Escherichia coli (E Coli) is the leading cause of community and hospital acquired urinary tract infections affecting approximate 150 million people each year worldwide. Uropathogenic Escherichia ... -
In-house expression and purification of phusion DNA polymerase for high fidelity polymerase chain reaction. (pcr) amplification
(Makerere University, 2022-04-05)Background: DNA polymerases are indispensable tools in the molecular biology field during synthesis of DNA molecules in the Polymerase Chain Reaction (PCR). Phusion DNA polymerase engineered from a processivity enhancing ... -
Urinary tract infections among male patients in Mulago National Referral Hospital.
(Makerere University, 2022-03-19)Background; Urinary Tract Infection is the type of infection that involves the any part of the urinary system including the kidneys, bladder, urethra ( Ahmet et al.,2015). Urinary Tract Infections (UTI) is known to affect ...